The publication focuses on the application of Atomic Force Microscopy (AFM) for the imaging and characterization of organic and polymer samples under various environmental conditions, such as humid air and solvent vapors (e.g., methanol, toluene, benzene, tetrahydrofuran). Utilizing the Agilent 5500 microscope, the study reveals how environmental factors induce structural transformations and swelling in samples, enhancing nanoscale characterization. Key findings include the reversible structural reorganization of block copolymers, humidity-driven molecular conformational changes, and the formation of self-assembled structures influenced by solvent vapors. The publication emphasizes the versatility of AFM in diverse environments and its ability to provide insights into molecular interactions, mechanical properties, and dielectric behaviors. These findings showcase AFM’s potential for advancing materials science by enabling detailed, environmentally controlled studies of surface phenomena and nanoscale properties.